Hygiene Atlas

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8. Load and sterilize

Also called: run the autoclave, load the sterilizer

Load so steam reaches everything, then run the cycle the manufacturer specifies.

Arrange the load so steam (or vapor or hot air) can circulate around every package: do not overload, and stand pouches on their edge. Run the time and temperature cycle the sterilizer's manufacturer specifies. All sterilizers used must be FDA-cleared medical sterilizers.

What you do

  1. Check the reservoir water level before the first cycle.
  2. Watch or record the cycle's time, temperature and pressure when it ends.

Safety and care

  1. Improper loading and overloading are common reasons for a failed spore test.

Related

Sources: CDC. Guidelines for Infection Control in Dental Health-Care Settings, 2003. MMWR 2003;52(RR-17), CDC. Guideline for Disinfection and Sterilization in Healthcare Facilities (2008): Steam Sterilization, Tuttnauer. Tabletop autoclave operation and maintenance manual, manual units (MAN205-0007000EN Rev. 27), CDC. Guideline for Disinfection and Sterilization in Healthcare Facilities (2008): Sterilizing Practices, CDC. Dental Infection Prevention FAQs: Sterilization Monitoring